Development and validation of ultraviolet spectroscopic method for estimation of methoxsalen in bulk using methanol and phosphate buffer (pH 7.4)

Abstract

Background: Psoriasis is an inflammatory condition of a body wherein erythematous, sharply demarcated papules and round-shaped plaques appear onto skin due to the hyperproliferation of epidermis. Psoralens are the class of furanocoumarins that are effectively used in the treatment of psoriasis and vitiligo in conjunction with exposure to a dose of ultraviolet radiation. It is crucial to develop and validate the suitable analytical method for its quantitative estimation. The present work aims to develop and validate simple, rapid, accurate and cost-effective (as compared to other sophisticated techniques of analysis) Ultraviolet-Visible Spectrophotometry in methanol and phosphate buffer saline (pH 7.4). Materials and Methods: Methanol and phosphate buffer saline (pH 7.4) were the two solvents chosen for method development and results of validation parameters were calculated. Results: Methoxsalen showed maximum absorbance at 249 nm in methanol and at 247 nm in phosphate buffer saline (pH 7.4). The percent recoveries for determination of accuracy and repeatability were found within a suitable confidence interval of 98 %-102 %. Conclusion: The developed method was evaluated quantitatively for accuracy, precision, robustness, ruggedness and other parameters.