Ion/ion reactions of multiply deprotonated peptide anions with xenon radical cations result in electron abstraction to generate charge-reduced peptide anions containing a free-radical site. Peptide backbone cleavage then occurs by hydrogen radical abstraction from a backbone amide N to facilitate cleavage of the adjacent CC bond, thereby producing a- and x-type product ions. Introduction of free-radical sites to multiply charged peptides allows access to new fragmentation pathways that are otherwise too costly (e.g., lowers activation energies). Further, ion/ion chemistry, namely electron transfer reactions, presents a rapid and efficient means of generating odd-electron multiply charged peptides; these reactions can be used for studying gas-phase chemistries and for peptide sequence analysis. © 2005 American Society for Mass Spectrometry.
CITATION STYLE
Coon, J. J., Shabanowitz, J., Hunt, D. F., & Syka, J. E. P. (2005). Electron transfer dissociation of peptide anions. Journal of the American Society for Mass Spectrometry, 16(6), 880–882. https://doi.org/10.1016/j.jasms.2005.01.015
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