Besides their well-known posttranscriptional effects on mRNA translation and decay, sRNAs and associated RNA chaperones (e.g., Hfq, CsrA) sometimes regulate gene expression at the transcriptional level. In this case, the sRNA-dependent machinery modulates the activity of the transcription termination factor Rho, a ring-shaped RNA translocase/helicase that dissociates transcription elongation complexes at specific loci of the bacterial genome. Here, we describe biochemical assays to detect Rho-dependent termination signals in genomic regions of interest and to assess the effects of sRNAs and/or associated RNA chaperones on such signals.
CITATION STYLE
Nadiras, C., Schwartz, A., Delaleau, M., & Boudvillain, M. (2018). Evaluating the effect of small RNAs and associated chaperones on rho-dependent termination of transcription in vitro. In Methods in Molecular Biology (Vol. 1737, pp. 99–118). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7634-8_7
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