NMR Investigation of Calmodulin

Abstract

Calmodulin (CaM) is a 16.7-kDa heat-stable protein that functions as Ca 2+ sensor in eukaryotes. Because of its physicochemical properties and its remarkable well-resolved NMR spectra for a helical protein, CaM has been extensively studied by a number of researchers for NMR methodology development as well as its biological appli-cations. This chapter describes the past and current NMR studies of CaM to serve as an example of structure and dynamic characterization of a protein.