Construction of a BALB/c congenic mouse, C.C3H-Lps(d), that expresses the Lps(d) allele: Analysis of chromosome 4 markers surrounding the Lps gene

Abstract

Development of a congenic BALB/c mouse strain that contains a segment of chromosome 4 including the Lps(d) allele of the lipopolysaccharide (LPS)- hyporesponsive C3H/HeJ strain is presented. On the basis of LPS-induced spleen cell mitogenesis, macrophage tumor necrosis factor secretion, and tyrosine phosphorylation in vitro and lethality in galactosamine-sensitized mice in vivo, the C.C3H-Lps(d) strain provides a model of LPS hyporesponsiveness that is comparable to that of the parental C3H/HeJ strain. Analysis of markers in this region indicates that length of the donor fragment is ~5.5 centimorgans. Thus, the C.C3H-Lps(d) strain provides an important genetic tool for analysis of markers in this region and for examining functional effects of Lps(d) expression on the BALB/c background.