Assessment of genetic diversity in Solanum torvum Swartz. From Tripura using SSR and RAPD markers

Abstract

Wild Solanum torvum shows widespread distribution throughout the state of Tripura. In this investigation, diversity analysis of five populations of S. torvum, each represented by single accession, from different geographical locations of Tripura has been carried out for the first time using two types of molecular markers, Simple Sequence Repeats (SSR) and Rapid Amplified Polymorphic DNA (RAPD). SSR markers revealed a high degree of polymorphism (86.38%) in comparison to RAPD primers (79.16%). Polymorphic information content ranged from 0.28 to 0.54 for SSR and 0.16 to 0.41 for RAPD, respectively. UPGMA cluster analyses based on molecular data grouped the genotypes into two different clusters. Our findings suggests that SSR has a high discriminatory power wherein it was observed that the genotype from Jampui hills (STP5) with greater genetic diversity is stabilized in the high altitude of Sub- Himalayan region of Tripura in the due course of evolution.