Escherichia coli extract-based cell-free expression system as an alternative for difficult-to-obtain protein biosynthesis

33Citations
Citations of this article
129Readers
Mendeley users who have this article in their library.

Abstract

Before utilization in biomedical diagnosis, therapeutic treatment, and biotechnology, the diverse variety of peptides and proteins must be preliminarily purified and thoroughly characterized. The recombinant DNA technology and heterologous protein expression have helped simplify the isolation of targeted polypeptides at high purity and their structure-function examinations. Recombinant protein expression in Escherichia coli, the most-established heterologous host organism, has been widely used to produce proteins of commercial and fundamental research interests. Nonetheless, many peptides/proteins are still difficult to express due to their ability to slow down cell growth or disrupt cellular metabolism. Besides, special modifications are often required for proper folding and activity of targeted proteins. The cell-free (CF) or in vitro recombinant protein synthesis system enables the production of such difficult-to-obtain molecules since it is possible to adjust reaction medium and there is no need to support cellular metabolism and viability. Here, we describe E. coli-based CF systems, the optimization steps done toward the development of highly productive and cost-effective CF methodology, and the modification of an in vitro approach required for difficult-to-obtain protein production.

Cite

CITATION STYLE

APA

Smolskaya, S., Logashina, Y. A., & Andreev, Y. A. (2020, February 1). Escherichia coli extract-based cell-free expression system as an alternative for difficult-to-obtain protein biosynthesis. International Journal of Molecular Sciences. MDPI AG. https://doi.org/10.3390/ijms21030928

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free