Photosynthesis and growth of Erythrina variegata as affected by water stress and triacontanol

Abstract

Erythrina variegata Lam. seedlings were grown under water stress (Ψ = -3.2 MPa) and subsequently sprayed with triacontanol (Tria). Water stress significantly reduced shoot growth rate, while roots continued to grow. Content of chlorophyll (Chl) a decreased more than that of Chl b. Water stress also reduced photosynthetic activity of chloroplasts as measured by Chl fluorescence induction. Stress effect was identified at the oxidation site of photosystem (PS) 2 prior to the hydroxylamine donating site and perhaps close to or after the diphenylcarbazide donating site. The loss of O2 evolving thylakoid polypeptides (33, 23, 17 kDa) and the large (55 kDa) and small (15 kDa) subunits of ribulose-1,5-bisphosphate carboxylase (RuBPC) were found in water stressed seedlings. The reduction in RuBPC activity was accompanied by reduction of CO2 fixation and stomatal conductance. All photosynthetic parameters were improved by Tria.