Diabetogenic Activity of Streptozotocin on Kunming Strain Mice as Animal Model of Diabetes Mellitus

Abstract

Diabetes mellitus (DM), a chronic metabolic disorder, is increasing tremendously around the world. Assessment of interventions designed to prevent disease progression in humans takes years. On the other hand, the use of plants as diabetic agent needs preclinical test to obtain scientific evidence. Therefore, appropriate experimental animal model of diabetes mellitus is essential tools for understanding the pathogenesis of diabetes mellitus and effectiveness of diabetes phytotherapy. Streptozotocin-induced diabetes is a well-documented model of experimental diabetes. The present study is aimed to investigate the diabetogenic activity of streptozotocin influenced by difference of doses, sex, administration route, duration induction and injection frequency on Kunming strain mice. Induction of STZ on Kunming mice were done according to experimental design and fasting blood glucose level measured using automatic glucometer, in which blood glucose more than 11.1mmol/L is considered as diabetes mellitus. These findings suggest that mild diabetes refers to type 2 diabetes mellitus when fasting blood glucose level is between 11.1-24.9mmol/L. It can be obtained by inducing male Kunming mice using STZ administrated either intravenously at dose of 100 mg/kg or intraperitoneally at dose of 180 mg/kg, in single injection for duration of 3 and 7 days, respectively.