Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA

Abstract

Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies Involve multiple steps that Introduce bias and Inefficiency. Here we apply a single-molecule approach to directly sequence chromatin lmmunopreclpltated DNA with minimal sample manipulation. This method Is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell populations. © 2010 Nature America, Inc. all rights reserved.