Recent improvements in technologies such as omics analysis have enabled us to acquire a large amount of data regarding the biological changes in cells treated with bioactive small molecules. Using such data, a variety of profiling methods have been established for target identification of such bioactive compounds. In this chapter, we describe a proteomic profiling system, ChemProteoBase, based on proteome analysis using two-dimensional difference gel electrophoresis. This system compares the similarities in protein expression of 296 spots detected in the gel among the test compounds.
CITATION STYLE
Muroi, M., & Osada, H. (2019). Proteomic profiling for target identification of biologically active small molecules using 2D DIGE. In Methods in Molecular Biology (Vol. 1888, pp. 127–139). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8891-4_7
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