Serological investigation of five diseases; influenza, newcastle disease, salmonella, mycoplasma gallisepticum and mycoplasma synoviae in native hens of Eghlid, Iran

Abstract

Aim: The study was conducted to determine seroprevalence of the five diseases influenza, Newcastle, Mycoplasma gallisepticum, Mycoplasma synoviae and salmonella, among around native hens of Eghlid in Iran, on spring 2011. Materials and Methods: On the basis of native Hens distribution, this region divided into four parts of Eghlid, Doskord, Sedeh and Hasan-abad. Fifty unvaccinated native Hens randomly selected from each part. Blood samples were aseptically collected from the wing veins using 5-ml sterile syringe. Serum from hens was tested for detection and titration for Mycoplasma and Salmonella by the rapid slide agglutination method, and was tested for influenza and Newcastle by the Hemagglutination Inhibition Assay. The data was analyzed completely in randomized design with four treatments, 50 repetitions for each disease. Results: 34 out of 200 samples (17%) were positive for influenza. There were significant differences between regions (p <0.01). 38 out of 200 samples (19%) were positive for Newcastle. The maximum infectious rate obtained from Eghlid. There were significant differences between regions (p <0.05). 170 out of 200 samples (85%) were positive for Mycoplasma gallisepticum. 4 from 200 samples (2%) were positive for Mycoplasma synoviae. The results do not show a significant difference for salmonella (p <0.05). Conclusion: Contamination of Influenza, Newcastle and Mycoplasma gallisepticum was high, and the highest contamination rate was related to Mycoplasma gallisepticum. It is usually recommended that preventive strategies, such as appropriate husbandry and hygiene, sanitary handling of chicks and eggs, routine health monitoring and vaccination of Native hens should be emphasized.