Gas chromatography-mass spectrometry (GC-MS) is considered the gold standard for analyzing and quantifying the presence of biological compounds in tissue samples due to its high sensitivity, peak resolution, and reproducibility. In this chapter, we describe a step-by-step modified Bligh and Dyer protocol for lipid extraction from the optic nerve tissue and a procedure for GC-MS analyses of the lipid extract. These protocols are based on our experience and can be modified depending on samples and compounds of interest.
CITATION STYLE
Sarohia, G. S., Garza, E. D., & Bhattacharya, S. K. (2019). Analyses of cholesterol metabolites of optic nerve using GC-MS methods. In Methods in Molecular Biology (Vol. 1996, pp. 47–51). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9488-5_5
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