Generation of arming yeasts with active proteins and peptides via cell surface display system: Cell surface engineering, Bio-arming technology

Abstract

The cell surface display system in yeast enables the innovative strategy for improving cellular functions in a wide range of applications such as biofuel production, bioremediation, synthesis of valuable chemicals, recovery of rare metal ions, development of biosensors, and high-throughput screening of proteins/ peptides library. Display of enzymes for polysaccharide degradation enables the construction of metabolically engineered whole-cell biocatalyst owing to the accessibility of the displayed enzymes to high-molecularweight polysaccharides. In addition, along with fl uorescence-based activity evaluation, fl uorescenceactivated cell sorting (FACS), and yeast cell chip, the cell surface display system is an effective molecular tool for high-throughput screening of mutated proteins/peptides library. In this article, we describe the methods for cell surface display of proteins/peptides of interest on yeast, evaluation of display effi ciency, and harvesting of the displayed proteins/peptides from cell surface.