In vitro and in vivo studies glycosylated gadolinum nanomagnetic particleas (GD-DTPA-DG) as new potential metabolic contrast agent in mMRI

Abstract

Early cancer diagnosis using MRI imaging is of high global interest as a noninvasive and powerful modality in molecular Imaging. Therefore demand for new MRI contrast agents, with an enhanced sensitivity and advanced functionalities that improve the targeting to specific tissues or organs, is very high. in thise study, D-glucose amine was conjugated to a well-known chelator, diethylenetriamine penta-acetic acid (DTPA), then labeled with Gd to achieve Gd-DTPA-DG, Which is a metabolic contrast agent in mMRI. The contrast agent was synthesized and characterized physicochemical using different techniques including dynamic light scattering (DLS), high resolution transmission electron microscopy (HTEM) and inductively coupled plasma atomic emission spectroscopy (ICP-AES). Efficacy of the targeted contrast agent was assessed by measuring relaxation rate in vitro and tumor MR imaging were performed to determine signal intensity (SI) in vivo (0.1 mmol Gd/kg) in female balb/c mice model. According to the results, the nano metabolic contrast agent penetrate into cells and accumulated in tumor, which cause improve the contrast of tumor tissue in comparison with magnevist. The results showed that the novel nano contrast agent could become useful tool in early detection of cancer.