Inhibition of microsomal prostaglandin E2synthase-1 as a molecular basis for the anti-inflammatory actions of boswellic acids from frankincense

Abstract

Background and Purpose Frankincense, the gum resin derived from Boswellia species, showed anti-inflammatory efficacy in animal models and in pilot clinical studies. Boswellic acids (BAs) are assumed to be responsible for these effects but their anti-inflammatory efficacy in vivo and their molecular modes of action are incompletely understood. Experimental Approach A protein fishing approach using immobilized BA and surface plasmon resonance (SPR) spectroscopy were used to reveal microsomal prostaglandin E2synthase-1 (mPGES1) as a BA-interacting protein. Cell-free and cell-based assays were applied to confirm the functional interference of BAs with mPGES1. Carrageenan-induced mouse paw oedema and rat pleurisy models were utilized to demonstrate the efficacy of defined BAs in vivo. Key Results Human mPGES1 from A549 cells or in vitro-translated human enzyme selectively bound to BA affinity matrices and SPR spectroscopy confirmed these interactions. BAs reversibly suppressed the transformation of prostaglandin (PG)H2to PGE2mediated by mPGES1 (IC50= 3-10 μM). Also, in intact A549 cells, BAs selectively inhibited PGE2generation and, in human whole blood, β-BA reduced lipopolysaccharide-induced PGE2biosynthesis without affecting formation of the COX-derived metabolites 6-keto PGF1αand thromboxane B2. Intraperitoneal or oral administration of β-BA (1 mg·kg-1) suppressed rat pleurisy, accompanied by impaired levels of PGE2and β-BA (1 mg·kg-1, given i.p.) also reduced mouse paw oedema, both induced by carrageenan. Conclusions and Implications Suppression of PGE2formation by BAs via interference with mPGES1 contribute to the anti-inflammatory effectiveness of BAs and of frankincense, and may constitute a biochemical basis for their anti-inflammatory properties. © 2010 The British Pharmacological Society.