Myocardial Effects of Halothane and Sevoflurane in Diabetic Rats

Abstract

Background: Diabetes induces significant myocardial abnormalities, but the effects of halogenated anesthetics on this diseased myocardium remain a matter of debate. Methods: Left ventricular papillary muscles and triton-skinned cardiac fibers were provided from control and streptozotocin-induced diabetic rats. The effects of halothane and sevoflurane were studied on inotropic and lusitropic responses, under low (isotony) and high (isometry) loads in papillary muscles and then on isometric tension-Ca2+ concentration (pCa) relations obtained in triton-skinned cardiac fibers. Data are presented as mean ± SD. Results: Sevoflurane and halothane induced a negative inotropic effect that was more important in diabetic rats (active force: 1.5% halothane, 19 ± 6 vs. 24 ± 6% of baseline, P < 0.05; 3.6% sevoflurane, 47 ± 14 vs. 69 ± 17% of baseline, P < 0.05). However, when differences in minimum alveolar concentration were considered, no significant difference was observed between groups for halothane. The effects of halothane and sevoflurane on isotonic relaxation and postrest potentiation were not significantly different between groups. In contrast, the decrease in Ca 2+ myofilament sensitivity produced by each anesthetic agent was greater in diabetic rats than in control rats (0.65% halothane, -0.15 ± 0.07 vs. -0.05 ± 0.04 pCa unit, P < 0.05; 1.8% sevoflurane, -0.12 ± 0.06 vs. -0.06 ± 0.04 pCa unit, P < 0.05). Conclusions: The negative inotropic effect of halothane and sevoflurane was greater in diabetic rats, mainly because of a significant decrease in myofilament Ca 2+ sensitivity.