Regeneration of plants from in vitro culture of petioles in Prunus domestica Lindl (European plum)

Abstract

Adventitious shoot regeneration from the petioles of European plum 'Tardicots' (Prunus domestica) was studied in this study. The influences of different concentrations of thidiazuron (TDZ) and 2,4-Dichlorophenoxyacetic acid (2,4-D), dark culture period, and different basal salts on shoot regeneration were evaluated. Woody plant medium (WPM) was the best basal medium for the European plum initial culture, supplemented with 2.0 mg L -1 thidiazuron (TDZ) and 0.2 mg L -1 2,4-D. The explants, which were maintained in darkness for 14 d, as a result had the highest regeneration rate (83.33%). The regenerated shoots were subcultured at 4 weeks intervals. Addition of IBA (1.0 mg L -1) in the rooting medium produced the highest roots (93.3%) when maintained in dark for 7 d.