Production and characterization of monoclonal antibodies to four Egtved virus structural proteins

Abstract

With the aim of studying molecular mechanisms of virulence and immunogenesis of Egtved virus, the causative agent of viral haemorrhagic septicaemia (VHS) of rainbow trout Salmo gairdneri , monoclonal antibodies (MAbs) were produced against 4 dominant virus proteins (G, N, M sub(1) and M sub(2)). The reactivity of each MAb was determined by ELISA, immunoblotting, immunofluorescence and plaque neutralization. Results are consistent with published information on the protein composition and cellular appearance pattern of other rhabdoviruses studied in vitro.