Obesity-induced lysine acetylation increases cardiac fatty acid oxidation and impairs insulin signalling

Abstract

Aims Lysine acetylation is a novel post-translational pathway that regulates the activities of enzymes involved in both fatty acid and glucose metabolism.We examined whether lysine acetylation controls heart glucose and fatty acid oxidation in highfat diet (HFD) obese and SIRT3 knockout (KO) mice. Methods and results C57BL/6 mice were placed on either a HFD(60% fat) or a low-fat diet (LFD; 4% fat) for 16 or 18weeks. Cardiac fatty acid oxidation rates were significantly increased in HFD vs. LFD mice (845±76 vs. 551±87 nmol/g dry wt min, P < 0.05). Activities of the fatty acid oxidation enzymes, long-chain acyl-CoA dehydrogenase (LCAD), and b-hydroxyacyl-CoA dehydrogenase (β-HAD) were increased in hearts from HFD vs. LFD mice, and were associated with LCAD and b-HAD hyperacetylation. Cardiac protein hyperacetylation in HFD-fed micewas associated with a decrease in SIRT3 expression, while expression of the mitochondrial acetylase, general control of amino acid synthesis 5 (GCN5)-like 1 (GCN5L1), did not change. Interestingly, SIRT3 deletion in mice also led to an increase in cardiac fatty acid oxidation compared with wildtype (WT) mice (422±29 vs. 291±17 nmol/g dry wt min, P < 0.05). Cardiac lysine acetylationwas increased in SIRT3 KOmice compared withWTmice, including increased acetylation and activity of LCADand b-HAD. Although the HFD and SIRT3 deletion decreased glucose oxidation, pyruvate dehydrogenase acetylation was unaltered.However, theHFD did increase Akt acetylation, while decreasing its phosphorylation and activity. Conclusion We conclude that increased cardiac fatty acid oxidation in response to high-fat feeding is controlled, in part, via the downregulation of SIRT3 and concomitant increased acetylation of mitochondrial β-oxidation enzymes. © The Author 2014.