Functional fluorescence microscopy of brain slices using voltage sensitive fluorescent proteins (VSFPs) allows large scale electrophysiological monitoring of neuronal excitation and inhibition. We describe the equipment and techniques needed to successfully record functional responses optical voltage signals from cells expressing a voltage indicator such as VSFP Butterfly 1.2. We also discuss the advantages of voltage imaging and the challenges it presents.
CITATION STYLE
Quicke, P., Barnes, S. J., & Knöpfel, T. (2017). Imaging of brain slices with a genetically encoded voltage indicator. In Methods in Molecular Biology (Vol. 1563, pp. 73–84). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6810-7_5
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