Simultaneous identification and quantification of nitrosylation sites by combination of biotin switch and ICAT Labeling

Abstract

S-nitrosylation is a widespread modification of proteins. In plants, most information available to date regarding this modification was obtained using nitric oxide donors and concerned the proteins but not the identification of cysteine residues specifically modified in the proteins or their quantification. Here, we describe a method for the identification of endogenously nitrosylated cysteines in Arabidopsis and, simultaneously, the measurement of relative change in their abundance within binary comparisons. © 2014 Springer Science+Business Media, LLC.