Involvement of ethylene biosynthesis and perception in the susceptibility of citrus fruits to Penicillium digitatum infection and the accumulation of defence-related mRNAs

Abstract

Citrus fruits infected with the fungus Penicillium digitatum substantially increase the production of the plant hormone ethylene. In this study, the regulation of ethylene biosynthesis in Citrus sinensis-infected fruits and its putative involvement in an active defence response against P. digitatum infection is examined. Ethylene production is demonstrated as being the result of the co-ordinated and differential up-regulation of at least three ethylene biosynthetic genes: ACS1, ACS2, and ACO. Blocking ethylene perception by 1-MCP resulted in an increased ethylene production and ACS2 expression during infection and mechanical wounding, suggesting that this gene is negatively regulated by ethylene. ACO expression was induced by ethylene in the absence of wounding or infection, although further results indicate that its induction during the course of infection may not be primarily mediated by ethylene. Treatment with 1-MCP also increased susceptibility to Penicillium decay, showing an involvement of ethylene perception in promoting defence responses in citrus fruits. The changes in the expression of two defence-related genes up-regulated during infection were also studied: the ones coding for phenylalanine ammonia-lyase (PAL) and an acidic class Il chitinase (ACR311). The onset of PAL expression after mechanical wounding or inoculation was not changed in 1-MCP-pretreated fruits, while its later increase during the course of infection was abolished. Chitinase gene induction was more related to mechanical damage and was partially repressed by ethylene. These studies indicate distinct possible regulatory mechanisms of plant fruit defence genes in the context of fungal infection and ethylene perception. © The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.