Gaussia luciferase-based mycoplasma detection assay in mammalian cell culture

Abstract

Mycoplasma contamination in mammalian cell culture is a common problem with serious consequences on experimental data, and yet many laboratories fail to perform regular testing. In this chapter, we describe a simple and sensitive mycoplasma detection assay based on the bioluminescent properties of the Gaussia luciferase reporter. © 2014 Springer Science+Business Media New York.