Isolation and in Vitro Translation of RNAs from Developing Peach Fruit

Abstract

Peach [ Prunus persica (L.) Batsch] RNA was extracted from fruit at six times during development, from leaves and from isolated ovules. These RNAs were translated in a wheat germ extract and the resulting polypeptides were compared by one-dimensional NaDodSO 4 /PoIyacrylamide gel electrophoresis (PAGE). RNAs encoding polypeptides of 100,85, 59,54,42, 41, and 25.5 kDa increased, while those encoding polypeptides of 46, 40, 25.5, and 25 kDa decreased during peach fruit development. These RNAs are present in fruit but not in ovules or leaves. This study suggests that fruit development in peach is at least partially regulated at the transcriptional level or at the level of mRNA stability.