Anti-cancer inhibition studies of naturally occurring Terpenes using in-vitro technique

Abstract

Solvent extractions of shade dried seeds of Cyamopsis tetragonoloba, Pithecellobium dulce and Coriandrum sativum plants were executed using organic solvents. The obtained extracted crudes were subjected to the chemical analysis followed by instrumental analysis using standard procedures to identify the naturally occurring phytochemicals [1, 2, 3,]. Limonene, Oleanolic acid and Linalool were obtained from ethanol extracts of Cyamopsistetragonoloba, methanol extract of Pithecellobium dulce and acetone extract of Coriandrumsativum respectively. The compound identification were confirmed by 1H NMR, 13C NMR, IR and Mass spectroscopy [4]. The anti-cancer activity of the extracted terpenes determined using in-vitro analysis on HeLa-Human cervix cell lines. The obtained IC50 value of Oleanolic acid shows better biological activity against HeLa cell lines. It was also found that there is no cell lysis in HeLa cell lines in case of Limonene with concentration less than 30 μg. However, biological activity of Linalool is better in case of HeLa cell line. This suggests that Oleanolic acid and Linalool is good anti-cancer agent as reported from in vitro studies against Hela-Human cervix cell line.