RNA interference (RNAi) refers to post-transcriptional silencing of gene expression as a result of the introduction of double-stranded RNA into cells. The application of RNAi in experimental systems has significantly accelerated elucidation of gene functions. In order to facilitate large-scale functional genomics studies using RNAi, several high-throughput approaches have been developed based on microarray or microwell assays. The recent establishment of large libraries of RNAi reagents combined with a variety of detection assays has further improved the performance of functional genome-wide screens in mammalian cells. © 2006 Springer-Verlag Berlin Heidelberg.
CITATION STYLE
Janitz, M., Vanhecke, D., & Lehrach, H. (2006). High-throughput RNA interference in functional genomics. Handbook of Experimental Pharmacology, 173, 97–104. https://doi.org/10.1007/3-540-27262-3_5
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