Abstract
RNA-interference enjoys a wide range of applications in medical and biological research. In particular, it is used to study functions of genes. One of the most popular approaches to this task is gene knockdown by small interfering RNA (siRNA). Currently there is no unified protocol for this method, which results in low reproducibility of experimental data. In the following article we outline the theoretical bases for this method and provide practical recommendations for its use in siRNA-mediated gene silencing experiments.
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Vyakhireva, J. V., Filatova, A. Y., Krivosheeva, I. A., & Skoblov, M. Y. (2017). SiRNA-mediated gene silencing. Bulletin of Russian State Medical University, 6(3), 17–29. https://doi.org/10.24075/brsmu.2017-03-02
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