FORMULATION OF GARGLE FROM TRIGONA SP. PROPOLIS EXTRACT AND ITS ACTIVITY AGAINST STREPTOCOCCUS MUTANS

Abstract

Objective: In this research, the formulations of a gargle with Trigona sp. propolis extracts as an alternative of dental caries therapy and test its activity against Streptococcus mutans was carried out. Methods: The experimental laboratory have been carried out with the following stages of work, material collection and processing, extraction of Trigona sp. propolis by maceration with 95% ethanol, phytochemical screening, determination of minimum inhibitory concentration, formulation of the gargle, activity, contact time and stability test of the formulas. Results: Phytochemical screening showed that Trigona sp. propolis extracts containing flavonoids, polyphenols, quinones, monoterpenoids and sesquiterpenoids. Minimum inhibitory concentration was 0.25% w/v. The formulation of gargle from Trigona sp. propolis extract were a yellow solution with mint odor and taste of mint and sweet. The antibacterial activity of gargle preparation and formulas from the market as a comparison, showed that the formula with a concentration of 1% w/v gave a significant difference to the market preparation. The formulation of gargle from Trigona sp. propolis extract has good inhibition against bacteria S. mutans with a contact time of 60 seconds and is physically stable during the 35 d of storage time. Conclusion: The formulation of gargle from Trigona sp. propolis extract is an excellent preparation to be developed in the future in the treatment of dental caries.