DNA microarray analysis

Abstract

The methods described in Chapter 9 are designed to look at the expression of specific selected genes in relatively small numbers. To ascertain mRNA changes that occur on a much larger scale microarray analyses can be performed. A microarray, also known as biochip by analogy with the computer industry terminology, can be described as the sum of a multitude of similar miniaturized assays that take place simultaneously on a small surface. Microarrays types include those for DNA, tissue, protein, and bacteria. DNA microarrays include those covering the whole genome, intergenic regions, and the coding regions of genes. Within this chapter we will focus on DNA microarrays with emphasis on arrays for analyzing gene expression. © 2009 Springer-Verlag US.