Suitable method for simultaneous and specific detection of maize (zea mays 1.) and genetically modified soyabean (glycine max 1.) in animal feeds

Abstract

Present method allows a simple and rapid identification of maize and specific transgenic soyabean event in raw materials and processed feed samples. The method combines two aspects: suitable DNA purification and amplification by means of multiplex RT-PCR. Efficiency and accuracy of this method have been tested and the limit of detection (LOD) reached was of 0.1 ng/μ1 for one of the species and of 1 ng/μ1 for the other one. The checking of the method has been realized by means of RT-PCR using the specific probes for each one of the systems by means of comparison with certified reference materials. The obtained results showed that with this method it is possible to obtain, in a shorter time, quality values for both: identification and quantification of GMOs, in raw and processed samples.