Obtaining photoperiod-sensitive genic male sterility (PGMS) lines is one of the most important steps in two-line hybrid rice breeding. Traditionally, such lines were screened and developed with a classic rice breeding system under both long-day and short-day conditions. The isolation and backcross process used for this could easily last for more than 3 years with a very low success rate. Here, we describe a straightforward method for generating csa-based PGMS lines by using the CRISPR-Cas9 technology in rice.
CITATION STYLE
Gu, W., Zhang, D., Qi, Y., & Yuan, Z. (2019). Generating photoperiod-sensitive genic male sterile rice lines with CRISPR/Cas9. In Methods in Molecular Biology (Vol. 1917, pp. 97–107). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8991-1_8
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