Intracellular sterol transport occurs largely by non-vesicular mechanisms in which sterol transport proteins extract sterol from one membrane and transfer it to another across the cytoplasm. Here we describe a suite of complementary assays to measure intracellular sterol transport in the model eukaryote Saccharomyces cerevisiae, as well as to quantify protein-mediated sterol transport between populations of vesicles in vitro. The in vivo assays can be adapted to study sterol transport in other cell types.
CITATION STYLE
Chauhan, N., Jentsch, J. A., & Menon, A. K. (2019). Measurement of intracellular sterol transport in yeast. In Methods in Molecular Biology (Vol. 1949, pp. 115–136). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9136-5_10
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