Prediction of cyclooxygenase inhibitory activity of Curcuma rhizome from chromatograms by multivariate analysis

Abstract

The potential use of partial least square regression (PLS-R) models for the prediction of biological activities of a herbal drug based on its liquid chromatography (LC) profile was verified using various extracts of Curcuma phaeocaulis and their cyclooxygenase-2 (COX-2) inhibitory activities as the model experiment. The correlation of practically measured inhibitory activities and predicted values by PLS-R analysis was quite good (correlation co-efficient=0.9935) and the possibility of transforming chromatographic information into a measure of biological activity was confirmed. In addition, furanodienone and curcumenol were identified as the major active anti-inflammatory constituents of C. phaeocaulis, through detailed analysis of the regression vector, followed by isolation of these compounds and their COX-2 inhibitory assays. The selectivity indices (SI), IC50 of COX-1/IC50 of COX-2, of both compounds were higher than that of indomethacin and it is considered that furanodienone and curcumenol are the most promising compounds as lead anti-inflammatory agents. © 2008 Pharmaceutical Society of Japan.