Immense addition of royal jelly apis mellifera (ceiba pentandra) insufficient to increase fibroblast preputium proliferation

Abstract

Culture of preputium skin fibroblasts is a good cell source in the manufacture of induced pluripotent stem cells (iPS). Induced pluripotent stem cells (induced pluripotent stem cell / iPSC) is the latest stem cell technology to get the source of stem cells that are equivalent to their pluripotent properties with embryonic cells, without having to consider ethical problems that arise. Culture systems of preputium skin fibroblast, can use a variety of media. Currently, Dulbeccos modified Eagle medium (DMEM) plus Fetal Bovine Serum (FBS), but if the culture is carried out continuously in FBS, it can increase the immune systems resistance. The addition of royal jelly 5 mg / ml of culture medium was not sufficient to increase the fibroblast preputium viability (p=0,05) so that further research to optimize FBS substitute culture medium is needed. This study was conducted to analyze the comparison of the effectiveness of serum free DMEM media with the addition of Apis Mellifera royal jelly bee from Ceiba pentandra flower for preputium skin fibroblasts cell culture. Royal jelly addition in 1%, 2%, and 5% showed less effect in the proliferation of fibroblast cell, compare with serum addition. The optimum medium for Fetal Bovine Serum (FBS) substitution in skin fibroblast cell cultures must continue to be developed, especially for the transduction protein medium. The results of this study are expected to be used to improve transduction protein medium in iPSC engineering.