Coelomic fluid of Lumbricus rubellus enhances anti-prolioniferative effect of 5-fluorouracil by modulating focal adhesion kinase express and IL-1β of colorectal cancer in mice

Abstract

The different malignancy advancing systems related with focal adhesion kinase (FAK) can be attentive in the progression of colorectal cancer. By inhibiting the growth mechanism of mixture of 5-Fluorouracil (5-FU) and earthworm therapy could enhance the affectability of malignant growth cells. The objective of this research was to explore the antiproliferative activity of mixed coelomic fluid cancer medicines produced from Lumbricus rubellus. Colorectal cancer was actuated in mice by injected HT-29 cells in the caecum of mice, and then 5% dextran sodium sulfate were given by the drinking water. To investigate the effect of combination treatment, we divided of five treatment groups: group 1 was untreated (vehicle), groups 2-5 were given by 5-FU. Groups 3-5 were given by a combination of 5-FU (50 μg/g BW) and coelomic fluid (50, 100, and 200 μg/g BW), respectively. The administrated of coelomic fluid was started from the 4th weeks. Mice were sacrificed at the end of 8 weeks and colon tissue was isolated, then assessed using Flowcytometry and immunofluorescent. These findings revealed that the mixture of 5FU and coelomic fluid components inhibits growth considerably, Interleukin-1β (IL-1β) and FAK compare with 5-FU only group (p < 0.001; p < 0.05; and p < 0.001). Treatment used coelomic fluid at a dose of 200 μg/g BW decreased FAK expression. The combination of 5-FU and coelomic fluid at a dose of 200 μg/g BW decreased the percentage of IL-1β (p < 0.05).