ESTUDIO PRELIMINAR DE EFECTOS ANTIMICROBIANOS “IN VITRO” DEL MUSGO Sphagnum magellanicum BRID

  • Wallach P
  • López L. L
  • Oberpaur C
  • et al.
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Abstract

Key words: Moss, antimicrobial, medicinal plants. Among the current tendencies in the study and use of medicinal plants, efforts are concentrated on the search for complexes that provide new active substances with antimicrobial activity. In the present study evaluations of the antimicrobial activity of Sphagnum magellanicum moss were carried out in 4 states: fresh, dried, sterilized and non-sterilized. In order to isolate the active components from the moss, different extractions were prepared using water, ethyl alcohol at 96%, acetone and hexane, and a process of rehydration, in the case of dried moss. The following bacteria and fungi were used as test microorganisms: Staphylococcus aureus, Streptococcus agalactiae, Bacillus cereus, Escherichia coli, Salmonella enteritidis, Pseudomona aeruginosa, Malassezia pachydermatis and Candida albicans. The selection of these microoganisms, was made so that Gram-positive bacteria, Gram-negative bacteria and yeast-like fungi groups would be included. The technique of Kirby-Bauer was used, a standard to measure sensitivity by diffusion in agar. The results showed antimicrobial activity only by Sphagnum moss in the fresh state and without being sterilized, extracted with acetone and hexane. The most sensitive microorganisms to the moss extract were the yeast-like fungi (which presented a greater diameter halo of inhibition), followed by Gram-positive bacteria and finally the Gram-negative bacteria. No effect was observed on P. aeruginosa. RESUMEN Palabras claves: Musgo, antimicrobiano, plantas medicinales. E n t r e l a s t e n d e n c i a s a c t u a l e s m á s generalizadas en el estudio y utilización de plantas medicinales se encuentran los esfuerzos que se realizan en la búsqueda de complejos que proporcionen nuevas sustancias activas con efectividad antimicrobiana. En el presente estudio se realizaron evaluaciones de la actividad antimicrobiana del musgo Sphagnum magellanicum (Pompón) en 4 estados; fresco, disecado, esterilizado y sin esterilizar. Para llegar al principio activo del musgo se realizaron distintas extracciones a partir de agua, alcohol etílico al 96%, acetona y hexano y un proceso de rehidratación con calor, para el caso del musgo disecado. Se emplearon para esta ocasión, como microorganismos de prueba, las siguientes bacterias y hongos: Staphylococcus aureus, Streptococcus agalactiae, Bacillus cereus, Escherichia coli, Salmonella enteritidis, Pseudomona aeruginosa, Malassezia pachydermatis y Candida albicans. La selección de las cepas antes mencionadas se realizó con la intención de tener representantes de los grupos de las bacterias Gram positivas, Gram negativas y hongos levaduriformes. El método utilizado fue la técnica de Kirby-Bauer, que es estándar para la realización de las pruebas de sensibilidad por difusión en agar. Los resultados obtenidos mostraron actividad antimicrobiana sólo del musgo en estado fresco *

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Wallach, P., López L., L., Oberpaur, C., Vacarezza, F., & Maier, L. (2010). ESTUDIO PRELIMINAR DE EFECTOS ANTIMICROBIANOS “IN VITRO” DEL MUSGO Sphagnum magellanicum BRID. Agro Sur, 38(2), 80–86. https://doi.org/10.4206/agrosur.2010.v38n2-03

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