M‐07e cell bioassay detects stromal cell production of granulocyte‐macrophage colony stimulating factor and stem cell factor in normal and in diamond‐blackfan anemia bone marrow

Abstract

Ten healthy donors and four patients with Diamond‐Blackfan anemia (DBA) have been investigated for granulocyte‐macrophage colony stimulating factor (GM‐CSF) and stem cell factor (SCF) production by bone marrow‐enriched fibroblasts (BMEF) in a highly sensitive biological assay on growth factor‐dependent M‐07e cells. M‐07e cells detected active soluble kit‐ligand from normal bone marrow fibroblasts as well as from DBA BMEF which produce constitutively significant amounts of SCF. Interleukin 1β (IL‐1β) induced a significant increase of soluble SCF from both normal and DBA BMEF. GM‐CSF was undetectable in unstimulated cultures, while its production by bone marrow microenvironmental cells was documented for both normal and DBA patients after IL‐1β stimulation in vitro. Copyright © 1993 AlphaMed Press