Distribution, Type, Accumulation, and Source of Marine Debris in the United States, 1989–1993

Abstract

Two rabbit erythrocyte casein kinases, GTP:casein kinase I and GTP:casein kinase II, have been purified 29 000- and 47 000-fold, respectively. Studies employing sucrose density gradient centrifugation indicate that kinase I has a molecular weight of about 9.5 · 105 (25 S) and kinase II about 1.4 · 106 (32 S). These enzymes can utilize either ATP or GTP as the phosphoryl donor. Among various protein substrates examined, these kinases catalyze the phosphorylation of casein >50% dephosphorylated phosvitin ≈50% dephosphorylated casein >phosvitin. Histones, protamine and bovine serum albumin are poor phosphryl acceptors. Kinetic data indicate that both enzymes are inhibited by high casein substrate concentrations which may be partially relieved by Nacl. Both phosphotransferases require Mg2+ for activity and are optimally active at ph 9.0. The enzymes have apparent Km values of 2.5 · 10-5 M for GTP, 2 · 10-5 M for ATP, and 0.4-0.6 mg/ml for casein. The incorporation of the terminal phosphate of GTP into casein as catalyzed by these enzymes is inhibited to varying degrees by ATP, ITP, ADP, and GDP but not by UTP, CTP, GMP, adenosine 3′:5′-cyclic monophosphate, and guanosine 3′:5′-cyclic monophosphate. In addition, NaF and 2,3-diphosphoglyceric acid are also found to inhibit the activity of both kinases. The effect of 2,3-diphosphoglycerate is interesting and suggests that this metabolite may regulate the activity of the casein kinases in the red blood cells. © 1975.