Taking transposable elements into consideration in surveys of genetic and epigenetic variation remains challenging in species lacking a high-quality reference genome. Here, molecular techniques reducing genome complexity and specifically targeting restructuring and methylation changes in TE genome fractions are described. In particular, methyl-sensitive transposon display (MSTD) uses isoschizomers and PCR amplifications to assess the methylation environment of TE insertions. MSTD offers reliable insights into genome-wide epigenetic changes associated with TEs, especially when used together with similar techniques tracking random sequences.
CITATION STYLE
Parisod, C. (2017). Profiling transposable elements and their epigenetic effects in non-model species. In Methods in Molecular Biology (Vol. 1456, pp. 243–250). Humana Press Inc. https://doi.org/10.1007/978-1-4899-7708-3_19
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