Gene expression analysis of the megakaryocytic lineage requires isolation of megakaryocytes from their bone marrow microenvironment. Laser microdissection of megakaryocytes from diagnostic bone marrow biopsies allows analysis of standardised formalin-fixed samples that reflect the in situ grown status quo of a physiological or pathological condition. Taking into account that in neoplastic proliferation, e.g. myeloproliferative neoplasms, non-neoplastic haematopoietic clones proliferate in parallel, this morphology-based isolation enables selective analysis of the aberrant megakaryocytic population. Two different laser microdissection devices are presented, and the details of RNA extraction and sub-sequent real-time qPCR gene expression analysis of mRNA and microRNA are provided.
CITATION STYLE
Hussein, K. (2011). Gene Expression Profiling in Laser-Microdissected Bone Marrow Megakaryocytes. In Methods in Molecular Biology (Vol. 755, pp. 429–439). Humana Press Inc. https://doi.org/10.1007/978-1-61779-163-5_36
Mendeley helps you to discover research relevant for your work.