Protein Microarrays: Overview, Applications and Challenges

Abstract

Microarrays technology represents a new tool to address high-throughputbiological studies. Various types of protein microarrays based on theapplication, format and content find use in basic research, drug andbiomarker discovery, as well as for the characterization of proteins. Inthis chapter, we discuss advantages and limitations of proteinmicroarrays, their features and recent applications. We also considerthe different methods to build protein microarrays and the recentadvances in cell free protein expression systems to construct in situprotein microarrays. Finally, we describe four types of self-assembledprotein microarrays: PISA (protein array to protein Array); DAPA (DNA toProtein Array); PuCa (in situ puromycin array) and NAPPA (Nucleic AcidsProgrammable Protein Arrays) and the recent applications of this latterin situ protein array.