The chromosomes in mammalian interphase nuclei are organized into domains called chromosome territories that play a major role in nuclear organization. Here we propose a methodology that combines the use of micro-patterning of adhesive molecules to impose single-cell geometry, with visualization of chromosome territories. This allows obtaining a representative statistical map of the absolute positions of chromosome territories relative to the geometry imposed to the cell population by combining the signal from each cell.
CITATION STYLE
Nastały, P., & Maiuri, P. (2021). Preparing map of chromosome territory distribution frequency. In Methods in Molecular Biology (Vol. 2157, pp. 213–219). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0664-3_12
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