Current single-cell RNA-seq approaches are hindered by preamplification bias, loss of strand of origin information, and the inability to observe small-RNA and mRNA dual transcriptomes. Here, we introduce a single-cell holo-transcriptome sequencing (Holo-Seq) that overcomes all three hurdles. Holo-Seq has the same quantitative accuracy and uniform coverage with a complete strand of origin information as bulk RNA-seq. Most importantly, Holo-Seq can simultaneously observe small RNAs and mRNAs in a single cell. Furthermore, we acquire small RNA and mRNA dual transcriptomes of 32 human hepatocellular carcinoma single cells, which display the genome-wide super-enhancer activity and hepatic neoplasm kinetics of these cells.
CITATION STYLE
Xiao, Z., Cheng, G., Jiao, Y., Pan, C., Li, R., Jia, D., … Jia, J. (2018). Holo-Seq: Single-cell sequencing of holo-transcriptome. Genome Biology, 19(1). https://doi.org/10.1186/s13059-018-1553-7
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