Western blotting is one of the most commonly used biochemical techniques to detect a specific protein from a mixture of proteins such as tissue extracts. Antibodies to the specific antigen are used to detect the protein. The mixture of proteins is resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a membrane. A specific antigen immobilized on the membrane is detected and visualized by a primary antibody, a secondary antibody-peroxidase conjugate, and a chemiluminescent reagent. © Humana Press Inc.
CITATION STYLE
Lee, C. (2007). Western blotting. Methods in Molecular Biology, 362, 391–399. https://doi.org/10.1385/1-59745-257-2:391
Mendeley helps you to discover research relevant for your work.