Enzyme-linked immunosorbent assay for detection of antibody to the hepatitis B surface antigen-associated delta antigen

Abstract

A blocking enzyme-linked immunosorbent assay (ELISA) was developed for specific detection of antibody to the hepatitis B surface antigen-associated δ antigen. The sensitivity of ELISA was intermediate between that of previously described immunofluorescence and radioimmunological assays for anti-δ. Performance of ELISA was simple, and required only ordinary and inexpensive laboratory equipment.