MicroRNAs (miRNAs) are small regulatory noncoding RNAs varying in length between 20 and 24 nucleotides. They play a key role during plant development by negatively regulating gene expression at the posttranscriptional level. Moreover, recent studies reported several miRNAs associated with abiotic stress responses. Small RNA cloning and high-throughput deep sequencing methods provide expression profiles of not only known miRNAs, but also novel miRNAs. In this chapter, we describe the methods used to identify and characterize abiotic stress-associated miRNAs and their target genes.
CITATION STYLE
Jeong, D. H., German, M. A., Rymarquis, L. A., Thatcher, S. R., & Green, P. J. (2010). Abiotic stress-associated miRNAs: detection and functional analysis. Methods in Molecular Biology (Clifton, N.J.), 592, 203–230. https://doi.org/10.1007/978-1-60327-005-2_14
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