Jeotgalicoccus halophilus sp. nov., isolated from salt lakes

Abstract

Two slightly halophilic bacterial strains, C1-52 T and YD-9, were isolated from Daban and Aiding salt lakes in Xinjiang, China, respectively. The isolates were Gram-positive, non-endosporeforming, non-motile, facultatively anaerobic cocci. Colonies were pale yellow, and a light pink, diffusible pigment was produced after a few additional days of incubation. The isolates grew optimally with 2-3% (w/v) NaCl, at pH 7.5 and at 30-35 6C. The peptidoglycan type was L-Lys- Gly 3-4-L-Ala(Gly). The menaquinones were MK-7 (83.2 %) and MK-6 (16.8 %). The major fatty acids (.10 %) were anteiso-C15: 0 and iso-C15: 0. The DNA G+C content of strains C1-52 T and YD-9 was 41.2 and 41.0 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains C1-52 T and YD-9 were closely related to Jeotgalicoccus psychrophilus YKJ-115T (98.0 and 97.1% 16S rRNA gene sequence similarity, respectively), followed by Jeotgalicoccus halotolerans YKJ-101 T (97.1 and 96.8 %). Strains C1-52 T and YD-9 shared, respectively, 20 and 11% DNA-DNA relatedness with J. halotolerans JCM 11198T and 8 and 13% with J. psychrophilus JCM 11199 T. DNA-DNA relatedness between the isolates was 91 %. On the basis of phenotypic and phylogenetic distinctiveness, strains C1-52 T and YD-9 belonged to the same species, which should be placed in the genus Jeotgalicoccus as a novel species. The name Jeotgalicoccus halophilus sp. nov. is proposed, with the type strain C1-52 T (5CGMCC 1.8911T 5NBRC 105788 T). © 2011 IUMS.