The Chloroplast Small Heat Shock Protein Studied by Peptide Mapping and Mass Spectrometry Using Purified Recombinant Protein From Arabidopsis Thaliana and Pea

Abstract

Purified recombinant heat shock protein (HSP) 21 oligomer subjected to oxidative stress became resistant to methionine-specific CNBr-cleavage, which indicated that the methionine residues had been oxidized to methionine sulfoxides. The HSP 21 oligomer also exhibited a conformational change when it became oxidized, which was evident as a changed mobility and as an alteration in its tryptic digest peptide pattern