Monitoring oncogenic B-RAF-induced senescence in melanocytes

Abstract

The B-RAF kinase is a downstream effector of the RAS family of proto-oncogenes and is constitutively activated in the majority of human melanomas. The common oncogenic B-RAF V600E mutant cooperates with additional genetic lesions to transform immortal murine and human cells. In primary cells, however, B-RAF V600E triggers a rapid cell cycle arrest that is phenotypically indistinguishable from cellular senescence. Here we describe the analyses of B-RAF-induced senescence in primary human melanocytes using recombinant lentiviruses. © Springer Science+Business Media New York 2013.